Hplc blood test

Background Thalassemia and hemoglobinopathies are heterogeneous group of inherited disorders that affects men and women equally. HPLC is a valuable method for hemoglobinopathy and/or thalassemia carrier screening. This study evaluate the role of cation exchange HPLC along with adjunctive tests as needed in the diagnosis of thalassaemias /haemoglobinopathies and to see the frequency of these disorders in the Iranian population. Methods This five-year study was conducted on 3780 patients. Initially complete blood count was done by autoanalyzer and then for detection of abnormal hemoglobins HPLC and HB electrophoresis methods was used. In cases with low MCV and MCH indices (MCV<80 fl,MCH<27 pg) and Hb-A2< 3.5% and normal Hb-electrophoresis, α-thalssemia trait(αα/--)was considered in the list of differential diagnosis. In cases with low MCV for exclusion of iron deficiency serum ferritin was meseared. Results Our rerults revealed that 1932 (51.11%) had normal electrophoretic pattern, 781 (20.66%) had β-thallasemia trait and 487(12.84%) had β-thallasemia major or intermedia,328( 8.67% ) had normal electrophoresis along with iron deficiency and 142 ( 3.75%) had normal Hb -electrophoresis and normal iron status but low MCV and MCH indices.We also identified 11(0.29%) with Alpha thalasemia variants Hb-H disease/alpha trait and 22(o.58%)with sickle trait and 18(o.47%)with sickle disease and 9(0.23%) S-Thal double heterozygote and 5(0.13%) with E- trait and 32(0.84%) with Hb-D varia...

Test Id Reporting Name Available Separately Always Performed HPFH Hb F Distribution, B No No MASS Hb Variant by Mass Spec, B No No SDEX Sickle Solubility, B Yes No IEF Isoelectric Focusing, B No No UNHB Hb Stability, B No No ATHAL Alpha-Globin Gene Analysis Yes No WASQR Alpha Globin Gene Sequencing, B Yes, (Order WASEQ) No WBSQR Beta Globin Gene Sequencing, B Yes, (Order WBSEQ) No WBDDR Beta Globin Cluster Locus Del/Dup,B Yes, (Order WBDD) No WGSQR Gamma Globin Full Gene Sequencing Yes, (Order WGSEQ) No HBEL0 Hb Electrophoresis Summary Interp No No This evaluation will always include hemoglobin (Hb) A2 and HbF and hemoglobin electrophoresis utilizing capillary electrophoresis and cation exchange high-performance liquid chromatography methods. Reflex testing, performed at additional charge, may include any or all of the following to identify rare hemoglobin variants present: sickle solubility (hemoglobin S screen); hemoglobin heat and isopropanol stability studies (unstable hemoglobin); isoelectric focusing, intact globin chain mass spectrometry (hemoglobin variant by mass spectrometry); HbF distribution by flow cytometry; DNA Sanger sequencing assays for: 1) beta-chain variants and the most common beta thalassemias (beta-globin gene sequencing), 2) alpha-chain variants and less common nondeletional alpha thalassemias (alpha-globin gene sequencing), or 3) gamma-chain variants and nondeletional hereditary persistence of fetal hemoglobin (HPFH) (gamma-globin full gene sequenc...

Citation Ludy Dobrila , Tracy Zhu , Dan Zamfir , Michal Tarnawski , Rodica Ciubotariu , Maria S Albano , Maryely DeLeon , Kelsey Turner , Mahin Azimi , Carolyn C. Hoppe , Andromachi Scaradavou , Pablo Rubinstein; Detection of Hemoglobin (Hb) Variants By HPLC Screening in Cord Blood Units (CBU) Donated to the National Cord Blood Program (NCBP). Blood 2016; 128 (22): 2182. doi: Download citation file: • • • • • • • • • Purpose. To evaluate the frequency and types of Hb abnormalities in CBU collected by the NCBP at the New York Blood Center. Background and Methods. Established in 1992, NCBP has collected and tested over 76,000 ethnically diverse CBU and manages an inventory of over 60,000 clinical grade CB products, including the first FDA-licensed (HEMACORD®). NCBP has released over 5,600 CBU for transplantation worldwide. To prevent transplants from donors affected with clinically significant hemoglobinopathy (HP), current Good Tissue Practices (cGTP) require Hb screening of all CBU before they are accepted to the public CB inventories. Clinically significant Hb disorders are caused by either structurally abnormal Hb variants (e.g., Hb S, Hb C, Hb E) or by decreased or absent production of α- or β-globin chains (thalassemia). Evaluation of NCBP CBU for possible HP includes: a) detailed family history for HP; b) complete blood count (CBC) and RBC indices, particularly mean corpuscular volume (MCV); c) screening using High-Performance Liquid Chromatography (HPLC) and d) confi...

Capillary electrophoresis (CE) is performed initially. If an abnormal hemoglobin is detected, appropriate testing will be performed at an additional charge. Depending on findings, one or more reflexive tests may be required in order to provide a clinical interpretation. These tests may include high-pressure liquid chromatography (HPLC) and/or hemoglobin solubility testing. 2 - 5 days Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary. • Hb A: – <7 months: Not established – 7 months to 1 year: 94.6% to 98.5% − ≥2 years : 96.4% to 98.8% • Hb F: − <7 months: Not established − 7 months to 1 year: 0.1% to 6.8% − ≥2 years : 0.0% to 2.0% • Hb S, D, G: 0% • Hb C, E, O: 0% • Hb A 2: – <7 months: Not established – 7 months to 1 year: 1.9% to 2.8% – ≥2 years: 1.8% to 3.2% • Hemoglobin solubility: Negative Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC 121690 Hgb Fractionation Cascade 43113-0 121692 Hgb F % 32682-7 121690 Hgb Fractionation Cascade 43113-0 121693 Hgb A % 20572-4 121690 Hgb Fractionation Cascade 43113-0 121694 Hgb A2 % 4552-6 121690 Hgb Fractionation Cascade 43113-0 121695 Hgb S % 32683-5 121690 Hgb Fractionation Cascade 43113-0 121696 Interpretation: 12710-0 Reflex Table for Interpretation: O...

What is a hemoglobin electrophoresis test? A hemoglobin electrophoresis test is a blood test used to measure and identify the different types of hemoglobin in your bloodstream. Hemoglobin is the protein inside red blood cells responsible for transporting oxygen to your tissues and organs. Genetic mutations can cause your body to produce hemoglobin that is formed incorrectly. This abnormal hemoglobin can cause too little oxygen to reach your tissues and organs. There are hundreds of different types of hemoglobin. They include: • Hemoglobin F : This is also known as fetal hemoglobin. It’s the type found in growing fetuses and newborns. It’s replaced with hemoglobin A soon after birth. • Hemoglobin A : This is also known as adult hemoglobin. It’s the most common type of hemoglobin. It’s found in healthy children and adults. • Hemoglobin C, D, E, M, and S : These are rare types of abnormal hemoglobin caused by genetic mutations. You acquire different abnormal types of hemoglobin by inheriting gene mutations on the genes that are responsible for producing hemoglobin. Your doctor may recommend a hemoglobin electrophoresis test to determine if you have a disorder that causes the production of abnormal hemoglobin. Reasons your doctor may want you to do a hemoglobin electrophoresis test include: 1. As part of a routine checkup: Your doctor may have your hemoglobin tested to follow up on a complete blood test during a routine physical. 2. To diagnose blood disorders: Your doctor may...

Hemoglobin electrophoresis is the process healthcare providers use to analyze hemoglobin in your red blood cells. Hemoglobin electrophoresis helps diagnose serious conditions like sickle cell anemia. It’s also one of several tests that screen newborn babies for sickle cell anemia and other rare but serious illnesses. Overview What is hemoglobin electrophoresis? Hemoglobin electrophoresis (pronounced he-ma-glow-bin elek-tro-fo-re-sus) is one process that healthcare providers use to analyze hemoglobin in your red blood cells. Hemoglobin is a protein in your red blood cells that helps cells carry oxygen throughout your body. Sometimes, the gene controlling your hemoglobin changes or mutates, turning healthy red blood cells into damaged cells. These damaged cells can break down and cause anemia and other Hemoglobin electrophoresis helps healthcare providers diagnose those conditions. This test is also one of several tests that screen newborn babies for signs of sickle cell anemia and other rare but serious illnesses. What’s the difference between a hemoglobin test and hemoglobin electrophoresis? What are the different hemoglobin types? Healthcare providers typically test for the four common hemoglobin types: • Hemoglobin Types A11 and A2 (HgbA1 and A2) : Hemoglobin A11 accounts for most of the hemoglobin in your normal red blood cells. Hemoglobin A2 accounts for about 2% to 3 % of your total hemoglobin. • Hemoglobin Type F (HgbF): Normally, this hemoglobin type accounts for th...